In Vitro Characterization of Borneol Metabolites by GC--MS Upon Incubation with Rat Liver Microsomes

Identification of In Vitro Metabolites of Amoxicillin in Human Liver Microsomes by LC–ESI/MS

Amoxicillin (AMOX) metabolism in human liver microsomes was studied in vitro using liquid chromatography-mass spectrometry (LC/MS). Amoxicillin was incubated with human liver microsomes along with NADPH, and the reaction mixture was analyzed by LC/MS to obtain the specific metabolic profile of the studied antibiotic drug. Positive electrospray ionization was employed as the ionization source. A...

Identification of stereoisomeric metabolites of meisoindigo in rat liver microsomes by achiral and chiral LC-MS/MS

Metabolism of Prazosin in Rat, Dog and Human Liver Microsomes and Cryopreserved Rat and Human Hepatocytes and Characterization of Metabolites by Lc/ms/ms

Prazosin, (2-[4-(2-furanoyl)-piperazin-1-yl]-4-amino-6,7-dimethoxyquinazoline), is an antihypertensive agent that was introduced to the market in 1976. It has since established an excellent safety record. However, in vitro metabolism of prazosin has not been investigated. This study describes the in vitro biotransformation of prazosin in liver microsomes from rats, dogs, and humans as well as r...

In Vitro Glucuronidation of Ochratoxin A by Rat Liver Microsomes

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and ...

In vitro identification of metabolites of verapamil in rat liver microsomes.

AIM To investigate the metabolism of verapamil at low concentrations in rat liver microsomes. METHODS Liver microsomes of Wistar rats were prepared using ultracentrifuge method. The in vitro metabolism of verapamil was studied with the rat liver microsomal incubation at concentration of 1.0 micromol/L and 5.0 micromol/L. The metabolites were separated and assayed by liquid chromatography-ion ...